Research Paper Volume 15, Issue 19 pp 10237—10252
Ginkgolide attenuates memory impairment and neuroinflammation by suppressing the NLRP3/caspase-1 pathway in Alzheimer’s disease
Figure 2. Detection of intracellular protein expression of ASC, NLRP3, and caspase-1 in BV-2 cells by western blotting. (A) Western blotting. Lane 1, control group (untreated); lane 2, Aβ group (treated with 2 μM Aβ1–42 for 12 h); lane 3, ginkgolide + Aβ group (pretreated with 25 μg/ml ginkgolide for 2 h followed by 2 μM Aβ1-42 for 10 h); lane 4, ginkgolide group (pretreated with 25 μg/ml ginkgolide for 12 h). (B–D) Effects of ginkgolide on intracellular protein expression of ASC, NLRP3, and caspase-1 in BV-2 cells. Data are presented as mean ± standard deviation. Abbreviations: Aβ: amyloid beta; ASC: apoptosis-associated speck-like protein containing a CARD; NLRP3: nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3.