Research Paper Volume 15, Issue 19 pp 10213—10236

Figure 4. ICT abrogates RANKL-induced NFATc1 and c-fos transcription and following osteoclast-related genes. BMMs were cultured in osteoclastogenic medium containing vehicle (DMSO) or ICT (1 μM) for 3 days. (A) The relative mRNA expression of NFATc1, c-fos, RANK, Cathepsin K, MMP9, TRAP. (B) Representative protein bands of NFATc1 and c-fos. (C) Quantitative analysis of NFATc1 and c-fos. (D) Representative protein bands of Cathepsin K and MMP9. (E) Quantitative analysis of Cathepsin K and MMP9. All data were presented as mean ± SD, n = 3, ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.