Research Paper Volume 15, Issue 19 pp 10213—10236

Figure 2. ICT inhibits osteoclast differentiation induced by RANKL. (A) Representative TRAP staining images of BMMs treated with DMSO or different doses of ICT in the presence of M-CSF and RANKL for 5 days. (B, C) Quantification of the number and area of TRAP-positive cells. (D) Representative TRAP staining images of BMMs treated with ICT in the presence of M-CSF and RANKL for the indicated time. (E, F) Quantification of the number and area of TRAP-positive cells. All data were presented as mean ± SD, n = 3. Abbreviation: ns: not statistical significance; ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.