Figure 9. Results of immunofluorescence and immunohistochemical staining and semi-quantitative analysis of femoral condylar defects. (A) Images of CD31 immunofluorescence staining and immunohistochemistry of CK1ε, OPN, and osteocalcin 4 weeks after surgery. (B) Semi quantitative analysis results of immunofluorescence and immunohistochemistry (n = 5), using ImageJ software. The one-way ANOVA was used for B. *P < 0.05; **P < 0.01; ***P < 0.001; Abbreviation: ns: not statistically significant.