Figure 3. Detection of mineralization after OD of BMSC. (A) The calcification status of different groups on the 7th day. (B) Qualitative analysis of ARS of different groups on the 7th day. (C) Detection of intracellular calcium levels after BMSC induction of different groups on the 7th day. (D) The calcification status of different groups on the 14th day. (E) Qualitative analysis of ARS of different groups on the 14th day. (F) Detection of intracellular calcium levels after BMSC induction of different groups on the 14th day. The experiments in this figure were repeated three times, and similar results were obtained. The one-way ANOVA was used for (B, C, E, F). *P < 0.05; **P < 0.01; ***P < 0.001; Abbreviation: ns: not statistically significant.