Research Paper Volume 15, Issue 19 pp 9913—9947

Figure 1. Effect of aging on endothelial cell DNA damage. (A) Representative images of immunofluorescence for the DNA damage marker 53BP1 on human microvascular lung endothelial cells from young (29 ± 1 year old, female) and old (67 ± 1 year old, female) donors. N = 3–4 experimental replicates from 2 young and 2 old donors. (B) Percentage of endothelial cells (EC) containing one or more 53BP1 foci. (C) Number of 53BP1 foci per endothelial cell. Experimental replicates on cells from the same donors are denoted by individual data points with like colors. (D) Representative images of immunofluorescence for the DNA damage marker 53BP1 on mouse microvascular lung endothelial cells from young (2.7 ± 0 mo old, male) and old (27 ± 0 mo old, male) mice. N = 5–8 experimental replicates from 12 young and 12 old mice per group. (E) Percentage of endothelial cells containing one or more 53BP1 foci. (F) Number of 53BP1 foci per endothelial cell. Individual data points with black borders denote females. Individual data points matching group colors denote males. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001. Scale bars are 10 μm.