Research Paper Volume 15, Issue 19 pp 10105—10116

Figure 7. SNHG25 promotes CRC metastasis by regulating MMP2 expression. (A) The migration and invasion abilities of HCT-116 cells were evaluated by transwell assays after indicated treatments. (B) Vimentin and E-Cadherin expression was detected by immunofluorescence in HCT-116 cells with indicated treatments. (C) MMP2, Vimentin and E-cadherin expression was detected by western blot in HCT-116 cells with indicated treatments. (D) Left panel, representative images of lungs from mice after tail vein injections with stably transfected sh-SNHG25 and sh-NC HCT-116 cells. Right panel, quantitative analysis of metastasis foci in corresponding groups. (E) Survival analysis of mice after tail vein injections with stably transfected sh-SNHG25 and sh-NC HCT-116 cells. (F) Schematic of the proposed mechanism of SNHG25 in CRC. *P < 0.05, **P < 0.01 and ***P < 0.001.