Research Paper Volume 15, Issue 19 pp 10105—10116

Figure 6. SNHG25 promoted MMP2 expression by adsorbing miR-296-3p. (A) Dual luciferase reporter assays with wild type or mutant MMP2-3’UTR luciferase reporters after transfection of miR-296-3p mimics. Upper panel, sequence alignment of miR-296-3p and its potential binding sites in MMP2 3’UTR. Predicted miR-296-3p target sequence (blue) in Luc-MMP2-3’UTR-Wt and mutated nucleotides (red) in Luc-MMP2-3’UTR-Mut. (B) MMP2 expression was detected by qRT-PCR after transfection of SNHG25 siRNAs or co-transfection of SNHG25 siRNAs and miR-296-3p inhibitors in HCT-116 cells. (C) MMP2 expression was detected by qRT-PCR after transfection of pcDNA3.1-SNHG25 or co-transfection of pcDNA3.1-SNHG25 and miR-296-3p mimics in HCT-116 cells. (D) MMP2 expression was detected by western blot in HCT-116 cells with indicated treatments. (E) qRT-PCR analysis of MMP2 expression in 90 pairs of CRC and corresponding adjacent normal tissues. (F) Correlation analysis between SNHG25, MMP2 and miR-296-3p in 90 paired CRC samples (miR-296-3p vs. SNHG25, R = 0.386, p < 0.001; miR-296-3p vs. MMP2, R = 0.492, p < 0.001; MMP2 vs. SNHG25, R = 0.519, p < 0.001). **P < 0.01 and ***P < 0.001.