Figure 4. Construction of m5C gene signature. (A) Univariate cox analysis in training set and (B) impact plots (from left to right, m5C isoforms, differential gene expression subtypes and m5C score classification and final sample survival, respectively), (C) KEGG enrichment for high and low scoring subgroups, (D) correlation between m5C score and hallmark pathway, (E) GO enrichment for high and low scoring subgroups, (F) immune checkpoint gene expression differences, (G) immune cell differences, (H) stromal score, immune score and tumor purity in high and low subgroups.