Figure 3.Eriocitrin treatment triggered ferroptosis in A549 and H1299 cells via suppressing Nrf2 expression. (A) Fluorescent images of ROS in A549 and H1299 cells. (B–F) Protein expression of ferroptosis-related genes after 50 μM concentration eriocitrin treatment, including SLC7A11, GPX4, Nrf-2 and FTH1. (G) Relative iron levels of A549 and H1299 cells after 50 μM concentration eriocitrin treatment. (H, I) mRNA expression of ferroptosis-related genes after 50 μM concentration eriocitrin treatment, including GPX4 and FTH1. *P < 0.05, **P < 0.01, and ***P < 0.001.
