Research Paper Volume 15, Issue 18 pp 9743—9758
NOTCH2 gene mutation and gamma-secretase inhibitor in mediating the malignancy of ovarian cancer
Figure 7. Expression of related proteins in A2780 and SKOV3 cells. (A, B) RT-PCR was used for evaluating IL-6 mRNA level. (C, D) WB analysis of p-Akt, Akt, Bcl-2, Bax, DNA-PK, Hes 1 and P65 proteins in OC cells. Each numerical value was the relative expression normalized to β-actin protein. (E, F) WB analysis of P65 proteins in the nucleus of OC cells. Each numerical value was the relative expression normalized to Lamin B protein. *p <0.05, **p <0.01, ***p <0.001. N=3.