Research Paper Volume 15, Issue 18 pp 9590—9613

Figure 3. YY1 facilitates ATG4B expression. (A) The analysis of the overlap (Venn diagram) between YY1 target genes and YY1-related genes revealing the four targets involved in the YY1 regulation of autophagy-related genes. (B) Heatmap showing the expression features of those four genes in GC and adjacent normal tissues from the Xena platform. (C) A correlation test validating the association between ATG4B and YY1 on the Xena platform. (D) qRT-PCR assay displaying the expression levels of ATG4B in GC and GES-1 cells. (E) ChIP assay unveiling YY1 enrichment on the ATG4B promoter in MGC803 and AGS cells stably transfected with Ca-NC, Ca-YY1, Ci-NC or Ci-YY1. (F) Schematic illustration showing the YY1 binding sites on the ATG4B promoter at -135/-121 bases from UCSC Genome Browser, and the wild-type (WT) or mutations (Mut) of ATG4B promoter reporters were designed. (G) Dual-luciferase assay disclosing the luciferase activity of ATG4B promoter in AGS and MGC803 cells stably transfected with Ca-NC, Ca-YY1, Ci-NC or Ci-YY1. (H, I) qRT-PCR and western blot assays showing the transcript and protein expression levels of ATG4B in MGC803 and AGS with YY1 overexpression or knockdown. ^*P < 0.05 vs. GES-1, Ca-NC, Ci-NC.