Research Paper Volume 15, Issue 18 pp 9572—9589

Figure 7. Knockout CF modification of TGF-βR I inhibits activation of TGF-β signaling pathway in vivo. (A–C) Representative immunofluorescence shows colocalization of TGF-βR I (Red) and FUT8 (Green) in different mice. The p-Smad2/3 expression in different treated mice was measured via immunofluorescence technique. Scale bar, 50 μm. Data were presented as the mean ± SD. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001. ^****P < 0.0001. (D) Immunoprecipitation of TGF-βR I with LCA was tested by immunoblotting analysis in different mice. (E, F) Western blotting using anti-p-Smad2/3 and anti-β-actin antibodies in different treated mice. Densitometric analyses of the bands were adjusted to be equal to β-actin. Data were presented as the mean ± SD. ^*P < 0.05. ^**P < 0.01.