Research Paper Volume 15, Issue 18 pp 9572—9589

Figure 6. CKO mice inhibit BLM-induced cellular senescence and ameliorate lung fibrosis in vivo. (A) CKO mice and Fl/Fl mice were treated with BLM for 21 days. HE and Masson staining reveal representative images of different lung tissue sections. Scale bar, 100 μm. (B–G) Western blotting using anti-FUT8, anti-E-cadherin, anti-collagen III, anti-p21, anti-p16, and anti-β-actin antibodies. Densitometric analyses of the bands were adjusted to be equal to β-actin. Data were presented as the mean ± SD. ^*P < 0.05. ^***P < 0.001. ^****P < 0.0001. (H–K) The protein levels of collagen I in different mice were measured via immunofluorescence technique. Representative immunofluorescence shows colocalization of p21^WAF1 or p16^ink4a (Red) and SPC (Green) in the lung tissue of different mice. Scale bar 50 μm. Data were presented as the mean ± SD. ^**P < 0.01. ^***P < 0.001. ^****P < 0.0001. (L) SA-β-gal staining reveals indicative pictures of different lung tissue sections. Scale bar, 100 μm. (M) RT-PCR revealed FUT8 and E-cadherin mRNAs expression in different groups. Data were presented as the mean ± SD. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001. (N) Hydroxyproline content of lung tissues. Data were presented as the mean ± SD. ^*P < 0.05. ^**P < 0.01.