Research Paper Volume 15, Issue 18 pp 9572—9589

Figure 2. CF modifications accompanying AECs senescence in pulmonary fibrosis. (A, B) SA-β-gal staining shows representative images of MLE₁₂ cells after 72 hours of BLM treatment. Scale bar, 10μm. Data were presented as the mean ± SD. ^**P < 0.01 compared to control (Unpaired t-test). (C–F) Western blotting using anti-FUT8, anti-p21, anti-p16, and anti-β-actin antibodies in MLE₁₂ cells treated with BLM or saline. The grayscale evaluations of the bands were adjusted to be equal to β-actin. Data were presented as the mean ± SD. ^*P < 0.05 compared to control (Unpaired t-test). ^**P < 0.01 compared to control (Unpaired t-test). (G–J) Representative immunofluorescence shows colocalization of p21^WAF1 or p16^ink4a (Red) and FUT8 (Green) in MLE₁₂ cells. DAPI was used to counterstain the nuclei. Scale bar, 25 μm. Data were presented as the mean ± SD. ^**P < 0.01 compared to control (Unpaired t-test). ^***P < 0.001 compared to control (Unpaired t-test). ^****P < 0.0001 compared to control (Unpaired t-test). (K–N) Immunofluorescence shows colocalization of p21^WAF1 or p16^ink4a (Red) and LCA (Green) in MLE₁₂ cells treated with BLM. DAPI was used to counterstain the nuclei. Scale bar, 25 μm. Data were presented as the mean ± SD. ^**P < 0.01 compared to control (Unpaired t-test). ^***P < 0.001 compared to control (Unpaired t-test). ^****P < 0.0001 compared to control (Unpaired t-test).