Research Paper Volume 15, Issue 19 pp 10057—10071

Figure 7. The regulation of the biological function of ccRCC cells by LINC00667-miR-143-3p-ZEB1 axis. Caki cells were transfected along with LINC00667 overexpression plasmids, miR-143-3p mimics and/or ZEB1 overexpression plasmids, while A498 cells were transfected together with LINC00667-in, miR-143-3p-in, and/or ZEB1-in. (A, B) LINC00667, miR-143-3p, and ZEB1 levels in Caki and A498 cells were tested via qRT-PCR. (C, D) Cell proliferation verified by CCK-8. (E, F) Migration and invasion in the cells were checked through Transwell. (G, H) Cell migration monitored by the wound healing test. Scale bar=100 μm. (I, J) Apoptosis gauged through TUNEL assay. Scale bar=50 μm. (K, L) The levels of apoptosis-concerned proteins were ascertained by WB. **P<0.01, ***P<0.001 (vs. NC/NC-in). nsP>0.05, ^^P<0.01, ^^^P<0.001 (vs. LINC00667 or LINC00667-in). nsP>0.05, #P<0.05, ##P<0.01, ###P<0.001 (vs. LINC00667+miR-143-3p or LINC00667-in+miR-143-3p-in). N=3.