Research Paper Volume 15, Issue 19 pp 10057—10071

Figure 3. Effects of overexpression of LINC00667 on ccRCC cells. (A, B) Caki and A498 were transfected together with si-LINC00667 or NC-in. The transfection validity of LINC00667 was checked by qRT-PCR. (C, D) Cell viability was measured through MTT assay. Caki and A498 cells transfected with si-LINC00667 or NC-in, and then were treated with DDP (5μg/mL) for 48 hours. (E, F) The migration and invasion of the cells were monitored through Transwell. Scale bar=100 μm. (G, H) Cell migration was evaluated by the wound healing test. (I, J) Apoptosis was gauged through TUNEL assay. Scale bar=50 μm. (K) Bad, Bax, and cleaved Caspase-3 levels were determined via WB. (L) EMT was assessed by determining E-cadherin, N-cadherin, and Vimentin alterations. *P<0.05, **P<0.01, ***P<0.001 (vs. NC). #P<0.05, ##P<0.01, ###P<0.001 (vs. DDP+NC). N=3.