Figure 2.Effects of overexpression of LINC00667 on ccRCC cells. (A, B) Caki and A498 were transfected together with LINC00667 overexpression plasmids. The transfection validity of LINC00667 was checked by qRT-PCR. (C, D) DDP (0, 5, 10, 15, 20 μg/mL) treated Caki and A498 cells for 48 hours. Cell viability was measured through MTT. Caki and A498 cells transfected with LINC00667 overexpression plasmids were treated with DDP (5μg/mL) for 48 hours. (E, F) Cell viability examined through MTT. (G, H) The migration and invasion activities of the cells monitored through Transwell. Scale bar=100 μm. (I, J) Cell migration evaluated by the wound healing test. (K, L) Apoptosis gauged through TUNEL. Scale bar=50 μm. (M) Bad, Bax, and cleaved Caspase-3 levels determined via WB. (N) EMT assessed through E-cadherin, N-cadherin, and Vimentin detection. **P<0.01, ***P<0.001 (vs. NC). #P<0.05, ##P<0.01 (vs. DDP+NC). N=3.
