Research Paper Volume 15, Issue 19 pp 10057—10071

Figure 2. Effects of overexpression of LINC00667 on ccRCC cells. (A, B) Caki and A498 were transfected together with LINC00667 overexpression plasmids. The transfection validity of LINC00667 was checked by qRT-PCR. (C, D) DDP (0, 5, 10, 15, 20 μg/mL) treated Caki and A498 cells for 48 hours. Cell viability was measured through MTT. Caki and A498 cells transfected with LINC00667 overexpression plasmids were treated with DDP (5μg/mL) for 48 hours. (E, F) Cell viability examined through MTT. (G, H) The migration and invasion activities of the cells monitored through Transwell. Scale bar=100 μm. (I, J) Cell migration evaluated by the wound healing test. (K, L) Apoptosis gauged through TUNEL. Scale bar=50 μm. (M) Bad, Bax, and cleaved Caspase-3 levels determined via WB. (N) EMT assessed through E-cadherin, N-cadherin, and Vimentin detection. **P<0.01, ***P<0.001 (vs. NC). #P<0.05, ##P<0.01 (vs. DDP+NC). N=3.