Research Paper Volume 15, Issue 17 pp 9167—9181

Figure 4. Doxorubicin-challenged H9c2 cells co-cultured with Jing Shi-preconditioned human adipose-derived stem cells (hADSCs) presented less hypertrophy and low-level mitochondrial reactive oxygen species. (A) F-actin staining detecting hypertrophy with or without Doxorubicin induction in H9c2 cells after co-culture with human adipose-derived stem cells (hADSCs). (B) quantitative analysis of cell area for Doxorubicin-challenged H9c2 cells. Jing Shi-preconditioned hADSCs significantly reduced hypertrophy in doxorubicin-challenged H9c2 cells (C, D) MitoSOX staining detecting mitochondrial reactive oxygen species and their quantitative analysis. Doxorubicin-challenged H9c2 cells showed the least mitochondrial reactive oxygen species levels after co-culture with Jing Shi-preconditioned hADSCs. Experiments were performed in triplicate. Data are presented as means ± SEM. *p <0.05, **p <0.01, and ***p <0.001 were significant.