Research Paper Volume 15, Issue 17 pp 9167—9181

Figure 1. Jing Shi-preconditioned human adipose-derived stem cells (hADSCs) enhanced cytoprotective effects of doxorubicin-challenged H9c2 cells. (A) Cell viability assay indicating cell viability of human adipose-derived stem cells (hADSCs) treated with Jing Shi. (B) Transwell migration assay showing that Jing Shi-preconditioned hADSCs showed more migration efficiency (pink color) compared with that of the control. (C) Schematic diagram outlining the strategy for co-culturing hADSC and doxorubicin-challenged H9c2 cells. (D) Immunoblot results showing that Jing Shi-preconditioned hADSCs co-cultured with doxorubicin-challenged H9c2 cells increased mTOR and CHIP expression and attenuated apoptosis marker p53 protein expression in H9c2 cells. Experiments were performed in triplicate. Data are presented as means ± SEM. *p <0.05, **p <0.01, and ***p <0.001 were considered significant.