Research Paper Volume 15, Issue 22 pp 12780—12793

Figure 4. TNRC6A is a direct target of miR-652-3p in human HCC cells. (A) The underlying targets of miR-652-3p were predicted using TargetScan and miRDB databases. (B) Expression of target genes in HepG2 cells determined by qRT-PCR after transfecting with miR-652-3p mimic. (C) Scheme and sequence of the intact miR-652-3p, TNRC6A (Wt) and its mutant (Mut). Computer prediction of miR-652-3p binding sites in the 3’UTR of human TNRC6A gene. (D) SMMC-7721 cells were co-transfected with miR-652-3p and WT or MUT 3’UTR of TNRC6A. Data were presented as the mean ± SD, and analyzed with Student’s t-test. ** < 0.01; compared with the indicated NC mimic controls. (E) Protein level of TNRC6A was detected by WB in HepG2 and SMMC-7721 cells transfected with NC mimic and miR-652-3p. GAPDH was also detected as a loading control. Data were presented as the mean ± SD, and analyzed with Student’s t-test; ** P < 0.01.