Research Paper Volume 15, Issue 17 pp 9041—9058
Galectin-3 promotes brain injury by modulating the phenotype of microglia via binding TLR-4 after intracerebral hemorrhage
Figure 1. The protein level of Gal-3 in microglia increased significantly at 24 h after ICH. (A) Brain tissue sections of rats in sham group and intracerebral hemorrhage group. (B) Experiment 1: the time course changes of Gal-3 after ICH. (C, D) Experiment 2: the role of Gal-3 in ICH - induced brain injury. (E, F) Western blot analysis and quantification of Gal-3 at 1h, 6h, 12h, 24h, 36h, 48h, 72h, and 168h after ICH. (G, H) Double immunofluorescence analysis of Gal-3(green) and different brain cells (red) in brain sections. microglial marker (Iba-1)/Neuron marker (MAP2)/ Astrocyte marker (GFAP). Nuclei were labeled with DAPI (blue). Arrow indicated Gal-3 positive cells. Scale bar =50 μm. The black dots represent individual data in each group. **p < 0.01 and *p < 0.05 vs. sham group, n = 6.