Figure 7.K591R mutant of DBC1 attenuates the interaction with p53. DBC1 KO cells were transiently transfected with DBC1-WT and DBC1-K591R mutant. (A) Their lysates were subjected to immunoprecipitation with anti-DBC1 antibody followed by immunoblot analysis as indicated. (B) Their lysates were subjected to immunoprecipitation with anti-p53 antibody followed by immunoblot analysis as indicated. Note that the K591R mutant of DBC1 weakens its interaction with p53.
