Research Paper Volume 15, Issue 16 pp 8501—8517
Xuebijing injection protects sepsis induced myocardial injury by mediating TLR4/NF-κB/IKKα and JAK2/STAT3 signaling pathways
Figure 4. Effects of XBJ on JAK2/STAT3 signaling pathway. (A) Representative images of western blotting for p-JAK2, JAK2, p-STAT3 and STAT3 in vivo (GAPDH was used as the internal reference protein). (B, C) Relative intensity of p-JAK2/JAK2 (B) and p-STAT3/STAT3 (C) was analyzed by western blotting. (D, E) Representative images of the protein expressions of p-JAK2 and p-STAT3 in myocardial tissue were detected by immunofluorescence localization. Positive p-JAK2 (D) and p-STAT3 (E) cells were stained green, with the sections counterstained with DAPI to visualize nuclei (blue). Data are expressed as mean ± SD (n=3/group), # indicates p<0.05 (vs. Sham group), * indicates p<0.05 (vs. CLP group).