Research Paper Volume 15, Issue 16 pp 8487—8500
ZBTB34 is a hepatocellular carcinoma-associated protein with a monopartite nuclear localization signal
Figure 6. The identification of importin α/β mediated ZBTB34. Co-immunoprecipitation with Western blot (anti-GFP antibody) technique was used to analyze the NLS of ZBTB34 bind to importin α1, importin α3, importin α4, importin α5, importin α7, or importin β1 in HepG2 cells. The results show pEGFP-ZBTB34-NLS is co-immunoprecipitated with importin α1, importin α3, importin α4, importin β1, but not with importin α5 or importin α7. The input was used as a control for protein detection.