Research Paper Volume 15, Issue 16 pp 8487—8500
ZBTB34 is a hepatocellular carcinoma-associated protein with a monopartite nuclear localization signal
Figure 3. The upstream and downstream regulators of ZBTB34. (A) ZBTB34 was identified as the target gene of miR-125b-5p using the online software TargetScan. qPCR analysis showed ZBTB34 expression was lower in the ago-miR-125b-5p group and higher in the antago-miR-125b-5p group. ZBTB34 expression is expressed as a fold change compared to control values normalized to 1. (B) We also used the online software UCSC (http://genome.ucsc.edu/) and STRING (https://cn.string-db.org/) to predict the putative genes that interact with ZBTB34. A total of eight genes were predicted and selected for further study. (C) ZBTB34 increased ZBTB10 and POLR1B expression while decreasing AUH expression. Fold changes in gene expression compared to control values normalized to 1.