Research Paper Volume 15, Issue 17 pp 8692—8711

Figure 4. APC is a target of IFNγ in suppressing spheroid proliferation. (A) SI organoid amount under treatment of IFNγ, and Notch signaling inhibitor IWP2 at different concentrations (2 μM, 20 μM). (B) Hypothesized network explaining IFNγ triggered GPX4-dependent ferroptosis. (C) SI organoid amount and Lgr5 (stem cell marker) gene expression under treatment of Wnt signaling inhibitor DKK1 at different concentrations (5 ng/mL, 10 ng/mL, 20 ng/mL, 50 ng/mL). (D) SI organoid amount and Lgr5 (stem cell marker) gene expression under treatment of ROCK inhibitor Y27632, apoptosis inhibitor Z-VAD-FMK and Z-DEVD-FMK, necrosis inhibitor GSK872, cell cycle checkpoint inhibitor YVDG. (E) SI organoid amount and quantification in response to IFNγ treatment (1 ng/mL, 5 ng/mL) when p53, p53/Kras, APC were knocked out respectively.