Research Paper Volume 15, Issue 16 pp 8444—8457
m6A methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression
Figure 2. METTL16 regulates the proliferation of CRC cells. (A) SW480 and SW620 cells were transfected with METTL16 overexpression vectors, and protein level of METTL16 was measured by western blotting assay. (B) SW480 and SW620 cells were transfected with METTL16 siRNAs, then protein level of METTL16 was measured by western blotting assay. (C) Cell viability of SW480 and SW620 cells under overexpression of METTL16 was measured by CCK-8 assay. (D) Proliferation of SW480 and SW620 cells under overexpression of METTL16 was detected by using colony formation assay. **p<0.01.