Research Paper Volume 15, Issue 16 pp 8258—8274

Figure 3. Acetyl-cinobufagin induced cell apoptosis in TNBC cells. (A) Hoechst 33342 staining and (B) flow cytometric analysis were performed to study the cell apoptosis rate after treatment with 0, 0.5, 1.0, and 2.0 μM of acetyl-cinobufagin for 24 h in MDA-MB-468, BT-549 and Hs578T cells by Annexin V and PI. (C, D) The expression levels of BAX and BCL-2 in above-mentioned three TNBC cell lines were determined by immunoblotting after treatment with acetyl-cinobufagin at three concentrations for 24 h. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared with the control using the Student’s t-test.