Figure 2.Acetyl-cinobufagin inhibits the migration of TNBC cells. (A) Wound healing assay was performed using the BT-549 cells with/without acetyl-cinobufagin treatment. (B) Transwell migration assay was performed with the MDA-MB-468, BT-549 and Hs578T cells. (C, D) Changes in the expression of EMT marker proteins (E-cadherin and N-cadherin) in Bt-549 cells were verified by immunofluorescence staining. (E) Expression of proteins related to the EMT signaling pathways as identified by Western blotting. *p < 0.05; **p < 0.01; ***p < 0.001 compared to the control as per Student’s t-test.
