Research Paper Volume 15, Issue 16 pp 8258—8274

Figure 1. Acetyl-cinobufagin inhibits the proliferation of TNBC cells. (A) Chemical composition of acetyl-cinobufagin. (B, C) The suppression rate was determined by the MTT assay in MDA-MB-468, BT-549, Hs578T cells. (D) The cinobufagin and acetyl-cinobufagin groups were intraperitoneally injected with 30 mg/kg on the 1^st day, and the death rate of the animals were recorded for 14 d. (E) Body weight curve of the animals. (F) Colony forming analysis was completed with the aforementioned three cell lines with/without acetyl-cinobufagin treatment. (G) Changes in the signals received by proteins (Ki67) in BT-549 cells were analyzed by immunofluorescence staining. Statistical analysis is illustrated as follows: (D) Log-rank (Mantel-Cox) test; (F) t-test. *p < 0.05; **p < 0.01.