Research Paper Volume 15, Issue 16 pp 8220—8236
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation
Figure 3. VCP regulates MM cell proliferation via modulating NF-κB signaling pathway. (A) Volcano plots showed the upregulated (red) and downregulated (blue) genes upon silencing VCP in MM cells. (B) KEGG pathway analysis of RNA-seq data indicated that VCP was associated with NF-κB signaling pathway. (C) WB analysis of P-P65 and P65 expressions in siVCP cells compared with NC cells. (D) WB analysis of P-P65 and P65 expressions in the cells treated with VCP20 compared with WT cells. (E) VCP20 increased IκBα expression. (F) The ubiquitination level of IKBα in MM cells with the treatment of VCP20 or not.