Research Paper Volume 15, Issue 16 pp 8220—8236
Inhibition of VCP modulates NF-κB signaling pathway to suppress multiple myeloma cell proliferation and osteoclast differentiation
Figure 2. VCP20 inhibits MM cell proliferation and induces cell apoptosis. (A) The structure of VCP20. (B) Predicted binding modes of VCP20 targeting VCP. (C) Inhibition ratio of VCP20 in MM cells. (D) Flow cytometry analysis indicated that VCP20 induced MM cell apoptosis. (E) WB analysis showed that VCP20 increased the expressions of apoptotic protein. (F) WB analysis showed that VCP20 decreased the CDK4/6 expression. The concentration of VCP20 was as follow: ARP1, 575.6 nM; H929, 661 nM. Data are presented as the mean ± SD; **p < 0.01; ***p < 0.001.