Coumarin-chalcone hybrid LM-021 and indole derivative NC009-1 targeting inflammation and oxidative stress to protect BE(2)-M17 cells against α-synuclein toxicity

Pei-Ning Yang; Wan-Ling Chen; Jun-Wei Lee; Chih-Hsin Lin; Yi-Ru Chen; Chung-Yin Lin; Wenwei Lin; Ching-Fa Yao; Yih-Ru Wu; Kuo-Hsuan Chang; Chiung-Mei Chen; Guey-Jen Lee-Chen

doi:doi:10.18632/aging.204954

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Research Paper Volume 15, Issue 16 pp 8061—8089

Coumarin-chalcone hybrid LM-021 and indole derivative NC009-1 targeting inflammation and oxidative stress to protect BE(2)-M17 cells against α-synuclein toxicity

Figure 6. Effects of LM-021 and NC009-1 on IL-1β- and IL-6-mediated inflammatory and GCLC- and GSTP1-mediated redox signaling in A53T-8 SNCA-GFP BE(2)-M17 cells. Western blot analysis of (A) NLRP1, NLRP3, ASC, IL-1β, (B) JNK (T183/Y185), JUN (S63), IκBα (S32/36), P65 (S536), P38 (T180/Y182), STAT1 (S727), (C) IL-6, JAK2 (Y1007/1008), STAT3 (Y705), and SOCS3, and (D) GCLC and GSTP1. GAPDH was used as an internal control (n = 3). For normalization, the NLRP1, NLRP3, ASC, IL-1β, JNK, JUN, IκBα, P65, P38, STAT1, IL-6, JAK2, STAT3, SOCS3, GCLC and GSTP1 in uninduced cells was set at 100%. P values: with vs. without doxycycline induction (^#: P < 0.05, ^##: P < 0.01 and ^###: P < 0.001), with vs. without fibril addition (^&: P < 0.05, ^&&: P < 0.01 and ^&&&: P < 0.001), or with vs. without compound treatment (*: P < 0.05, **: P < 0.01 and ***: P < 0.001).