Research Paper Volume 15, Issue 16 pp 8061—8089

Figure 3. Inflammation in BV-2 microglia induced by α-synuclein. (A) Experimental flow chart. BV-2 microglia were plated in 1% FBS on day 1. After 20 h, the cells were pretreated with the test compound (10 μM) for 8 h, and then treated with α-synuclein fibrils (2 μM). 20 h later, the BV-2 cells were analyzed for (B) morphology, MHC-II expression, (C) NO/IL-1β/IL-6/TNF-α release into culture medium, and (D) cellular NLRP3/CASP1/iNOS/IL-1β/IL-6/TNF-α expression (n = 3). For normalization, the relative MHC-II level in inactive cells or NLRP3/CASP1/iNOS/IL-1β/IL-6/TNF-α level in activated cells was set as 100%. P values: inactivated vs. activated cells (^##: P < 0.01, ^###: P < 0.001), or with vs. without treatment of compound (*: P < 0.05, **: P < 0.01, ***: P < 0.001).