Research Paper Volume 15, Issue 18 pp 9377—9390
Ar-turmerone inhibits the proliferation and mobility of glioma by downregulating cathepsin B
Figure 6. Overexpressing CTSB reversed the inhibitory effects of ar-turmerone on glioma cell proliferation and mobility. Glioma cells were treated with DMSO, ar-turmerone, CTSB plasmids or ar-turmerone + CTSB plasmids, respectively. (A) CCK−8 was used to detect the proliferative rate of glioma cells in each group. (B) A colony formation assay was used to detect the colony formation of glioma cells in each group. (C) A wound healing assay was used to detect the migration of glioma cells in each group. (D) A Transwell assay was used to detect the invasion of glioma cells in each group. (E, F) Subcutaneous tumorigenesis experiments were used to detect the proliferation of tumor tissues in each group. (G) Tumor weights of each group. *P < 0.05, **P < 0.01.