Research Paper Volume 15, Issue 18 pp 9377—9390

Figure 4. CTSB was identified as a key target of ar-turmerone. (A) Differentially expressed genes were identified in glioma cells treated with DMSO or ar-turmerone. (B) Heatmap showing changes in the expression of CTS family members in U251 cells. (C) qRT−PCR was used to detect the mRNA levels of CTSH, CTSO, CTSF, CTSK, CTSS, CTSV and CTSB in glioma cells treated with ar-turmerone (200 μM) or the control. (D) CTSB levels were significantly elevated in glioma tissues from The Cancer Genome Atlas database. (E) CTSB levels were significantly elevated in glioma tissues from our research cohort. (F) qRT−PCR was used to detect the expression of CTSB in normal human astrocytes, U251, U87 and LN229 cells. (G) The binding mode of ar-turmerone with CTSB, and the 3D illustration of the details of the interaction. Ar-turmerone is shown in blue, while the CTSB protein is shown in red. ^*P < 0.05; ^**P < 0.01.