Research Paper Volume 15, Issue 15 pp 7709—7726

Figure 4. BACE1 impaired protective phenotype transition of microglia in vitro. (A) Quantification of cytokine gene expression is shown in the bar graphs, with data from at least 5 independent experiments. Data are shown as the relative change of the Ctrl group, ^*P < 0.05 and ^**P < 0.01. (B) Secreted IL-10 and IL-1β protein levels in the vehicle- and MK-8931-treated PMG measured by ELISAs. (C) Representative images of in vitro erythrophagocytosis of PMG, scale bar = 20 μm. (D) The percentage of erythrophagocytosis was calculated in the indicated treated PMG. (E, F) In vitro analysis of PMG erythrophagocytosis under Hb stimulation with/without MK-8901 treatment. The phagocytosis index was calculated as the median fluorescence intensity of the RBC-engulfed population, n = 3 per group. All data are presented as the mean ± SD. ^*P < 0.05, ^**P < 0.01.