Research Paper Volume 15, Issue 15 pp 7673—7688

Figure 6. The RSL-3 induced cellular ferroptosis was suppressed by the treatment of Emodin. Different dose of RSL-3 was used to treat HK-2 cells exposed to culture medium containing 5.5 mmol/L glucose for 24 h (A). The mRNA expression of ferroptosis related indicators (B–F). The expression levels as well as semi-quantitative analysis of ferroptosis related proteins in RSL-3 group with or without Emodin treatment (G, H). The concentration of MDA and iron in RSL-3 group with or without Emodin treatment (I, J). ^*P < 0.05, ^**P < 0.01 vs. Ctrl group, ^#P < 0.05, ^##P < 0.01 vs. RSL-3 group.