Research Paper Volume 15, Issue 15 pp 7616—7636

Figure 10. OTUD6B−AS1 was associated with aggressive pathological parameters and promoted breast cancer progression. Expression of OTUD6B−AS1 was positively correlated with axillary lymph node metastasis (A), larger tumor size (B), advanced tumor stage (C, one way ANOVA, P = 0.0096; stage I vs. II, P = 0.0050; stage I vs. III, P = 0.0162; stage II vs. III, not significant), and high Ki-67 expression (D). The OTUD6B−AS1 overexpression plasmid significantly upregulated OTUD6B−AS1 expression in breast cancer BT474 cells (E). OTUD6B−AS1 promoted breast cancer cell proliferation (F), wound healing (G, 40× magnification), migration, and invasion (H, I, 200× magnification). OTUD6B−AS1 increased the tube formation ability of HUVEC (J, 40× magnification). OTUD6B−AS1 decreased E-cadherin expression and increased the expression of HIF-1α, MMP1, SMAD5, Snail, Twist1, and VEGFA (K, cropped gels blots are used).