Research Paper Volume 15, Issue 15 pp 7533—7550
HBx promotes hepatocellular carcinoma progression by repressing the transcription level of miR-187-5p
Figure 7. HBx promoted FoxP3 expression by E2F1. (A) Used PROMO website to predict the promoter of FoxP3. (B) E2F1 binding site in the FoxP3 promoter region. (C, D) Luciferase reporter assay in HEK-293T cells showed E2F1 significantly inhibited the luciferase activity of the vector carrying promoter of FoxP3, compared with control vector. (E) E2F1 significantly elevated the protein expression of FoxP3 by western blotting and reduced the expression of miR-187-5p by qRT-PCR. (F) HBx promoted the expression of E2F1 by western blotting. The experiments were repeated at least 3 independent times.