Research Paper Volume 15, Issue 15 pp 7533—7550

Figure 6. FoxP3 bound to promoter of miR-187-5p. (A) FoxP3 overexpression significantly decreased the expression level of miR-187-5p by RT-qPCR. (B) The FoxP3 binding site in the miR-187-5p promoter region. (C, D) Luciferase reporter assay in HEK-293T cells showed miR-187-5p significantly inhibited the luciferase activity of the vector carrying 3′-UTR of CDH2, compared with control vector. (E) FoxP3 significantly promoted the expression of CDH2 by western blotting. (F) Overexpression of FoxP3 promoted the proliferation of Huh-7 cells through EdU assays. (G) Overexpression FoxP3 increased the rate of wound closure. (H) In Transwell assays, results demonstrated more invasion number of hepatoma cells transfected with FoxP3, compared with control. The experiments were repeated at least 3 independent times. (F–H) Data was presented as mean ± SD from three independent experiments. ^*P < 0.05; ^**P < 0.01; ^***p < 0.001.