Research Paper Volume 15, Issue 15 pp 7533—7550
HBx promotes hepatocellular carcinoma progression by repressing the transcription level of miR-187-5p
Figure 5. miR-187-5p bound to 3’-UTR of CDH2. (A) The putative seed recognition site in the 3′-UTR of CDH2 for miR-187-5p. (B) Luciferase reporter assay in HEK-293T cells. miR-187-5p significantly inhibited the luciferase activity of vector carrying 3′-UTR of CDH2, compared with control vector. (C) Regulation of CDH2 expression by miR-187-5p with western blotting. The experiments were repeated at least 3 independent times. *P < 0.05; **P < 0.01.