Study on the effect of a triple cancer treatment of propolis, thermal cycling-hyperthermia, and low-intensity ultrasound on PANC-1 cells

Yu-Yi Kuo; Wei-Ting Chen; Guan-Bo Lin; Chueh-Hsuan Lu; Chih-Yu Chao

doi:doi:10.18632/aging.204916

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Research Paper Volume 15, Issue 15 pp 7496—7512

Study on the effect of a triple cancer treatment of propolis, thermal cycling-hyperthermia, and low-intensity ultrasound on PANC-1 cells

Figure 3. Triple treatment increased apoptosis on PANC-1 cells via mitochondrial dysfunction. (A) Apoptosis after treatment was analyzed via flow cytometry with Annexin V-FITC/PI double staining, and (B) the apoptotic percentage (Q2 + Q4) was calculated. (C) MMP level after treatment was analyzed via flow cytometry with DiOC₆(3) staining, and (D) the percentage of cells with the loss of MMP (M1) was calculated. Data were presented as the mean ± standard deviation in triplicate. ^**P < 0.01, ^***P < 0.001, comparison between indicated groups.