Research Paper Volume 15, Issue 14 pp 7308—7323
Subcellular localization of nucleolar protein 14 and its proliferative function mediated by miR-17-5p and E2F4 in pancreatic cancer
Figure 2. Impacts of dysregulation of NOP14 on pancreatic cancer cell proliferation. (A) Immunofluorescence analysis of NOP14 up- or downregulation in PANC1 pancreatic cancer cells. Magnification 100×. Blue, DAPI-labeled nuclei; green, FITC-conjugated anti-NOP14 antibody; red, phalloidin-labeled cytoskeletal F-actin; merge, superimposition of the above three images. (B) Proportion of NOP14-positive cells after up- or downregulation of NOP14, as assessed by fluorescence microscopy. (C) NOP14 expression level after up- or downregulation in PANC1 cancer cells, as determined by Western blot analysis with standardized relative gray ratio. (D) Effect of NOP14 inhibition on cell proliferation ability. (E) Effect of NOP14 overexpression on cell proliferation ability. *P < 0.05, **P < 0.01.