Research Paper Volume 15, Issue 15 pp 7440—7450

Figure 4. Both miR-590-5p and SATB1 were the downstream targets for hsa_circ_0010889. (A) Prediction of binding sites of miR-590-5p in hsa_circ_0010889. The MUT version of hsa_circ_0010889 is presented. (B) Relative luciferase activity determined 48 h after transfection of LN229 cells with miR-590-5p mimic/NC or hsa_circ_0010889 WT/Mut. Data are presented as means ± SD. ^***P < 0.001. (C) Prediction of binding sites of miR-590-5p within the 3′UTR of SATB1. The MUT version of the 3′-UTR-SATB1 is shown. (D) Relative luciferase activity determined 48 h after transfection of LN229 cells with miR-590-5p mimic/NC or 3′UTR-SATB1 WT/Mut. Data are presented as means ± SD. ^***P < 0.001. (E–J) RT-qPCR detection showing the expression of hsa_circ_0010889, miR-590-5p and SATB1 in both LN229 and U251 after transfection with si-hsa_circ_0010889, miR-590-5p inhibitor and SATB1 overexpression vector together or single. Data are presented as means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. NC. ^##P < 0.01, ^###P < 0.001 vs. si-hsa_circ_0010889.