Research Paper Volume 15, Issue 14 pp 7187—7218

Figure 9. Upregulation of ADAMTS2 alleviates the effect of TGF-β1 on cardiac fibroblasts. (A) The mRNA expression of Adamts2 in CFs and NRCMs was determined by RT-qPCR. * P < 0.05. (B) The protein expression of Adamts2 in CFs and NRCMs was determined western blot. * P < 0.05. (C) The mRNA expression of Adamts2 in TGF-β1 treated CFs at different time points. * P < 0.05 vs. the 0h group. (D) The protein expression of Adamts2 in TGF-β1 treated CFs at different time points. * P < 0.05 vs. the 0h group. (E) The overexpression of Adamts2 in CFs was determined by western blot. (F) The migration ability of CFs was showed by wound healing assay. (G) The relative migration distance of Adamts2 overexpressed CFs. (H) The cell viability was calculated by CCK-8. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β group. (I) The expression of α-SMA and Col1 were determined by western blot. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β group. (J) The downregulation of Adamts2 in CFs was determined by western blot. (K) The migration ability of CFs was showed by wound healing assay. (L) The relative migration distance of Adamts2 downregulated CFs. (M) The cell viability was calculated by CCK-8. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β group. (N) The expression of α-SMA and Col1 were determined by western blot. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β group.