Research Paper Volume 15, Issue 14 pp 7187—7218
Identification of TGF-β-related genes in cardiac hypertrophy and heart failure based on single cell RNA sequencing
Figure 11. The regulatory effect of TGF-β-MYC-ADAMTS2 axis on CFs. (A) The migration ability of CFs was showed by wound healing assay. (B) The relative migration distance of CFs. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+oe-NC+si-Adamts2 group. (C) The cell viability was calculated by CCK-8. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+oe-NC+si-Adamts2 group. (D) The expression of α-SMA and Col1 was determined by western blot. (E) The relative protein expression of α-SMA and Col. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+oe-NC+si-Adamts2 group. (F) The migration ability of CFs was showed by wound healing assay. (G) The relative migration distance of CFs. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+si-NC+oe-Adamts2 group. (H) The cell viability was calculated by CCK-8. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+si-NC+oe-Adamts2 group. (I) The expression of α-SMA and Col1 was determined by western blot. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+si-NC+oe-Adamts2 group. (J) The relative protein expression of α-SMA and Col. * P < 0.05 vs. the Control group. # P < 0.05 vs. the TGF-β1+si-NC+oe-Adamts2 group. (K) The ridgeplot for the GESA results of ADAMTS2. (L) The PI3K-Akt signaling pathway for ADAMTS2. (M) The MAPK signaling pathway for ADAMTS2.