Research Paper Volume 15, Issue 15 pp 7424—7439
DNA methylation-mediated high expression of CCDC50 correlates with poor prognosis and hepatocellular carcinoma progression
Figure 10. CCDC50 promotes the proliferation, migration, and invasion of LIHC cells. (A) The expression of CCDC50 in LIHC cell lines was examined via qRT-PCR assay. (B, C) The establishment of CCDC50 knockdown cell lines in Hep3B and Huh7 was verified via qRT-PCR assay and western blot. (D) The knockdown of CCDC50 dramatically inhibits the proliferation of Hep3B and Huh7 cells, examined via cell counting kit-8 assay. (E) The knockdown of CCDC50 dramatically inhibits the migration and invasion abilities of Hep3B and Huh7 cells. Data are presented as the mean ± SD of three independent experiments. **p < 0.01, ***p < 0.001.