Priority Research Paper Volume 15, Issue 13 pp 5966—5989

Figure 4. Transcriptomic rejuvenation by reprogramming small molecule cocktails. (A, B) Delta transcriptomic ages (tAgeΔ), as measured by a biological transcriptomic clock built on rodent and human transcriptomic data (A), or rodent data alone (B). (C) Delta ages, as measured by a chronological transcriptomic clock built using human data. n.s.: p > 0.05; ^*p < 0.05; ^**p < 0.01; ^***p < 0.001. (D) Correlation matrix of transcriptomic differences by cocktail treatment, iPSC reprogramming, or aging. ^*p-adj < 0.05; ^**p-adj < 0.01; ^***p-adj < 0.001. Benjamini-Hochberg approach. (E) Enrichment of pathways by cocktail treatment, iPSC reprogramming, or aging. Normalized Enrichment Score (NES) 0.05 < ^p-adj < 0.1; ^*p-adj < 0.05; ^**p-adj < 0.01; ^***p-adj < 0.001. Benjamini-Hochberg approach. (F) Pictograph of study results showing that both induction of OSK and treatment with C1-6 restore NCC integrity, transcript profiles, and reversing biomarkers of health. Created using https://www.biorender.com.